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Flow-Enhanced Single Molecule DNA Hybridization Studies

Objective: To develop novel microfluidic flow cells that allow trapping of single DNA molecules and studies of the binding of sequence-specific probes to the trapped DNA.

Fig. 2Approach: Two different devices have been designed and fabricated: a cross-slot that uses flow focusing to direct probes to the trapped DNA and a microfluidic "four roll mill" that allows the flow type to be varied from extension to shear to rotation near the trapped DNA (fig. 2).

Results & Impact: We have demonstrated the ability to make probes, trap DNA-probe complexes, and determine probe positions. We have used the four roll mill to investigate DNA tumbling dynamics. Our approach may ultimately allow fast and inexpensive single molecule genotyping.

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