In this paper we have reported development and evaluation of a miniaturized, inexpensive and battery operated ultra-sensitive nanomaterial based fluorescence resonance energy transfer (NSET) probe shown in Figure 1.
Contamination of the environment with mercury has been an important concern throughout the world for decades. Human exposure to high Hg levels can harm the brain, heart, kidneys, lungs, and immune system of people of all ages.
This represents the first nanoparticle-based fluorescence assay for probing single-strand DNA nuclease activity. Our simple assay with extremely high sensitivity will make it widely applicable and highly useful for convenient characterization of DNA/RNA cleavage reactions Given the simplicity, speed, and sensitivity of this approach, the described methodology could easily be extended to a high throughput format and become a new method of choice in HIV proteases and matrix metalloproteases (MMP) as well as the proteins involved in cellular apoptosis
One of the long-standing challenges is the development of organic compounds, which exhibits a large value of the 2PA cross section at wavelength(s) above 700 nm. We have designed a series of D-A Zn-porphyrin aggregates that possess exceptionally large Two Photon Absorption (TPA) cross sections at the desirable fundamental wavelengths of 1.1-1.5 m, which can be highly suitable candidates for the applications in biological imaging and photon dynamic therapy.